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Title   파라핀 포매된 비강 NK / T 세포 림프종 조직에서 DOP - PCR 과 Comparative Genomic Hybridization 을 이용한 염색체 이상 분석 ( Detormination of Chromosomal Alterations in Nasal NK / T - cell Lymphomas by DOP - PCR and Comparative Genomic Hybridization )
Publicationinfo   2000 Jan; 032(03): 578-587.
Key_word   Chromosomal alterations , Nasal NK / T cell lymphoma , Comparative genomic hybridization
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Abstract   Purpose: Because of difficulty of obtaining metaphase cells from tumor specimens, there are only a few cytogenetic studies in nasal NK/T-cell lymphomas, and so far no consistent specific chromosomal abnormalities have been described. In this study, we have used degenerate oligonucleotide primed PCR (DOP-PCR) and comparative genomic hybridization (CGH) to deter-mine chromosomal alterations from 6 nasal NK/T-cell lymphoma tissues dissected from formalin-fixed paraffin-embedded slide sections, Materials and Methods: For the isolation of tumor DNA, four 7- μm-thick tissue sections from each sample were dewaxed and rehydrated, and areas of high tumor cell content (more than 60%) were dissected and pooled into a tube, Normal DNA was prepared from the peripheral blood of a healthy volunteer, Tumor DNA was labeled with biotin-16-dUTP by DOP-PCR and normal DNA was labeled with digoxigenin-dUTP using a nick translation kit. In CGH, equal amounts of differently labeled DNA from the tumors and normal reference DNA were hybridized simultaneously to normal metaphase chromosomes. They were visualized by different fluorochromes, and the signal intensities were quantitated separately as gray levels for each chromosome. The over- and underrepresented DNA segments were determined by computation of image ratios and average ratio profiles. Results: Our results show that gains of DNA copy number were more prevalence than DNA losses. The most commonly observed gains were mapped to chromosomal regions of 1p32.2ter.
저 자   박상진(Sang Jin Park),하만준(Mahn Joon Ha),김현주(Hyon Ju Kim),박광화(Kwang Hwa Park),김현수(Hyun Soo Kim),양우익(Woo Ik Yang),김효철(Hugh Chul Kim)